When I was installing the “PASA” on my mac, there was an error with the perl module: “DBD::mysql”. I could not install it by CPAN. After looking for some advice from internet, I found this “Python mysqldb“, and it worked.
- Create a symbolic link in
sudo ln -s /usr/local/mysql/lib/libmysqlclient.18.dylib /usr/lib/libmysqlclient.18.dylib
Here is a blog post related to the RNA-seq question. More thoughts on the TruSeq RNA Sample Prep Kit. The author used the platform of illumina GA and found there was s wired problem with “per base sequence content”. After looking for some reference, he got the conclusion:
Continue reading “RNA-seq with illumina”
A short summary from StackExchange
Roche/454 FLX Pyrosequencer technology is based on pyrosequencing method, which utilizes the use of the enzymes ATP sulfurylase and luciferase. After the incorporation of each nucleotide by DNA polymerase, a pyrophosphate is released, which further takes part in downstream light-producing reactions. Continue reading “What is the difference between SOLiD, 454, and Illumina next-gen sequencing?”
一樣用一句話去去解釋 mate-paired 和 paired-end 的差異：mate-paired 技術可以讓定序兩端的間隔高達 2k-5k (目前新 kit 可到 10k )。如下圖所示： Continue reading “Mate-paired vs Pried-end sequencing and Optical mappin”